Prep HPLC Column, HPS Silica, 5um, 120A. 21.2mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Info:
75001-P50 Cogent HPS Silica™ Prep HPLC Column is packed with high‑purity, unmodified silica, delivering consistent and reproducible performance for normal phase purification of polar and hydrophilic compounds.
Because the stationary phase is not chemically modified, it provides strong surface interactions with polar analytes, making it highly effective for preparative purification of lipids, fats, and other hydrophilic compounds.
This proprietary HPS Silica™ phase has been widely used by HPLC laboratories for many years as a premium-value alternative to established silica products such as Inertsil® and Luna®, offering dependable selectivity and long-term performance.
The 5 µm particle size is ideal for preparative work, providing:
Lower backpressure compared to smaller particles
Higher flow rate capability
Robust performance under repeated purification cycles
50 mm Length – Fast Preparative Purification
The 50 mm length is optimized for high-throughput preparative workflows.
Very fast purification cycles
Reduced solvent consumption
Ideal for method scouting and early-stage scale‑up
✅ Recommended for rapid purification and high-throughput preparative workflows
21.2 mm ID – Preparative Scale
Designed for preparative HPLC purification
High sample loading capacity
Suitable for scale‑up from analytical and semi‑prep methods
Compatible with large‑volume injections and fraction collection systems
✅ Recommended for process development and production-scale purification
Flow Rate & System Requirements (21.2 mm ID, 5 µm)
⚠️ Requires preparative or process‑scale HPLC system capability
Typical flow rate range: ~15 – 40 mL/min
Low-to-moderate backpressure
Designed for high‑flow purification workflows
Compatible with preparative detectors and fraction collectors
✅ Important: A specialized preparative HPLC system is required for columns of this size
Why Choose HPS Silica™ for Preparative HPLC
High‑Purity Unmodified Silica
Strong retention of polar compounds
Ideal for normal phase purification
USP L3 Compatibility
Suitable for compendial and validated methods
Reliable alternative to legacy silica columns
Proven Performance
Widely used in preparative laboratories
Consistent, reproducible separations
Performance Advantages
Low Backpressure (5 µm Particles)
Enables higher flow rates for faster purification
Compatible with preparative systems
Fast Equilibration
Reduces cycle time between runs
Strong Polar Retention
Ideal for hydrophilic compounds, lipids, and fats
Typical Applications
Preparative purification of lipids and fats
Normal phase separations
Polar compound isolation
Process scale‑up from analytical HPLC
Pharmaceutical and chemical purification workflows
Important Usage Notes
Designed for normal phase chromatography conditions
Use appropriate non‑polar mobile phases
Requires preparative or process‑scale HPLC system capability
Ensure proper equilibration for reproducible results
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable